Composite

Part:BBa_K4115039:Design

Designed by: Kaijun Wang   Group: iGEM22_ShanghaiTech_China   (2022-09-30)


J23101-B0034-luxR-B0015-Lux pR-B0034-sfGFP-B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985


Design Notes

The function of LuxR is first verified in E. coli, which will then be used in A.caulinodans ORS571 to build a communication and regulation system between E. coli and S.elongatus HL7942.

Source

The sequence of LuxR is gained from the iGEM parts (BBa_C0062). The LuxR and sfGFP fragments were synthesized by the company. The plasmid was constructed by our team.

References